Crystal Violet 25G
Crystal violet (C.I. 42555) for microscopy Certistain®. CAS 548-62-9, pH 2.5 - 3.5 (10 g/l, H₂O, 20 °C).
Manufacturer: Merck
Catalog ID: 115940.0025
Stock: In Stock
Description
| Catalogue Number | 115940 |
| Synonyms | Hexamethylenepararosaniline chloride, Gentian violet (C.I. 42555), Hexamethyl-p-rosanilinium, Methyl violet 10 B |
| Description | CRYSTAL VIOLET (C.I. 42555) FOR MICROSCO |
| Overview | Crystal violet (C.I. 42555) is a CERTISTAIN® dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. The CERTISTAIN® dry dye can be used for clinical diagnostic purposes as it is certified and registered as IVD and CE product. |
Product Information
| CAS number | 548-62-9 |
| EC index number | 612-204-00-2 |
| EC number | 208-953-6 |
| Hill Formula | C₂₅H₃₀ClN₃ |
| Molar Mass | 407.99 g/mol |
| HS Code | 3204 13 00 |
Applications
| Application | Crystal violet (C.I. 42555) for microscopy Certistain®. CAS 548-62-9, pH 2.5 - 3.5 (10 g/l, H₂O, 20 °C). |
Physicochemical Information
| Density | 1.19 g/cm3 (20 °C) |
| Melting Point | 189 - 194 °C |
| pH value | 2.5 - 3.5 (10 g/l, H₂O, 20 °C) |
| Bulk density | 220 - 400 kg/m3 |
| Solubility | 10 g/l |
Toxicological Information
| LD 50 oral | LD50 Rat 420 mg/kg |
Storage and Shipping Information
| Storage | Store at +5°C to +30°C. |
Specifications
| Dye content (spectrophotometrically) | ≥ 88 % |
| Identity (UV/VIS-Spectrum) | passes test |
| Absorption maximum λ max (water) | 589 - 594 nm |
| Spec. Absorptivity A 1%/1cm (λmax; 0.002 g/l; water) | 2050 - 2450 |
| TLC-Test | passes test |
| Michler`s ketone | < 0.10 % |
| Loss on drying (110 °C) | ≤ 10 % |
| Suitability for microscopy | passes test |
