| Growth promotion test according to ISO 11133 Mixed inoculum of Listeria, E.coli and E.faecalis Additionaly monoculture of E.coli and E.faecalis Pre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser Broth Confirmation on ALOA®-Medium resp. Tryptic soy agar |
|
| Inoculum on reference medium - Listeria monocytogenes ATCC 19111 (WDCM 00020) |
10 - 100 |
| - Listeria monocytogenes ATCC 13932 (WDCM 00021) |
10 - 100 |
| - Escherichia coli ATCC 25922 (WDCM 00013) |
≥ 1000 |
| - Enterococcus faecalis ATCC 19433 (WDCM 00009) |
≥ 1000 |
| - Enterococcus faecalis ATCC 29212 (WDCM 00087) |
≥ 1000 |
| Blacking - Listeria monocytogenes ATCC 19111 (WDCM 00020) |
+ |
| - Listeria monocytogenes ATCC 13932 (WDCM 00021) |
+ |
| - Escherichia coli ATCC 25922 (WDCM 00013) |
- |
| - Escherichia coli ATCC 8739 (WDCM 00012) |
- |
| - Enterococcus faecalis ATCC 29212 (WDCM 00087) |
- |
| Growth on ALOA® medium - Listeria monocytogenes ATCC 19111 (WDCM 00020) |
> 10 CFU |
| - Listeria monocytogenes ATCC 13932 (WDCM 00021) |
> 10 CFU |
| Colonies - Listeria monocytogenes ATCC 19111 (WDCM 00020) |
bluegreen with non transparent zone |
| - Listeria monocytogenes ATCC 13932 (WDCM 00021) |
bluegreen with non transparent zone |
| Growth on Tryptic soy agar - Escherichia coli ATCC 25922 (WDCM 00013) |
no growth |
| - Escherichia coli ATCC 8739 (WDCM 00012) |
no growth |
| - Enterococcus faecalis ATCC 29212 (WDCM 00087) |
< 100 CFU |
|
< 100 CFU |
| Incubation: Half Fraser Broth: 24 hours at 30°C, aerobic Fraser Broth: 48 hours at 37°C, aerobic Confirmation on ALOA®-Medium: 48 hours at 37°C, aerobic Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic |
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