Gram's Crystal Violet Soln 500ml

for the Gram staining method

Manufacturer: Merck

Catalog ID: 109218.0500

Stock: In Stock

 

Description

Catalogue Number 109218
Description GRAM'S CRYSTAL VIOLET SOLUTION FOR THE G
Overview The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange. A bottle with 500 ml Gram's crystal violet solution is sufficient for staining up to 250 slides. Gram's crystal violet solution is also available as part of the Gram-color stain set. It is an IVD product and CE registered.

Product Information

HS Code 3204 19 00

Physicochemical Information

Density 0.99 g/cm3 (20 °C)
Flash point 47 °C

Storage and Shipping Information

Storage Store at +15°C to +25°C.

Specifications

Suitability for microscopy passes test
gram-positve microorganisms blue-violet
gram-negative microorganisms pink to red
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