Gram's Crystal Violet Soln 500ml
for the Gram staining method
Manufacturer: Merck
Catalog ID: 109218.0500
Stock: In Stock
Description
| Catalogue Number | 109218 |
| Description | GRAM'S CRYSTAL VIOLET SOLUTION FOR THE G |
| Overview | The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange. A bottle with 500 ml Gram's crystal violet solution is sufficient for staining up to 250 slides. Gram's crystal violet solution is also available as part of the Gram-color stain set. It is an IVD product and CE registered. |
Product Information
| HS Code | 3204 19 00 |
Physicochemical Information
| Density | 0.99 g/cm3 (20 °C) |
| Flash point | 47 °C |
Storage and Shipping Information
| Storage | Store at +15°C to +25°C. |
Specifications
| Suitability for microscopy | passes test |
| gram-positve microorganisms | blue-violet |
| gram-negative microorganisms | pink to red |