Insulin Receptor (β-Subunit) ELISA Kit
Manufacturer: Merck
Catalog ID: CBA039
Stock: In Stock
Description
| Overview | This product has been discontinued. Detects and quantifies the levels of insulin receptor (IR) β-subunit independent of its phosphorylation status. The IR is a cell surface receptor that belongs to the superfamily of the growth factor receptor tyrosine kinases and regulates multiple signaling pathways. Although this kit is designed for use with human cell lines, it cross-reacts with mouse and rat cells. |
| Catalogue Number | CBA039 |
| Brand Family | Calbiochem® |
| Materials Required but Not Delivered | Plate reader capable of measurement at or near 450 nm. • Calibrated adjustable precision pipettes, preferably with disposable plastic tips. (A manifold multi-channel pipette is desirable for large assays.) • Cell extraction buffer (see Recommended Formulation, p. 4). • Deionized or distilled H2O. • Plate washer: automated or manual (squirt bottle, manifold dispenser, etc.). • Graph paper: linear (Cartesian), log-log, or semi-log, as desired. • Glass or plastic tubes for diluting and aliquoting standard. • Absorbent paper towels. • Calibrated beakers and graduated cylinders in various sizes. |
Product Information
| Detection method | Colorimetric |
| Form | 96 Tests |
| Format | 96-well plate |
| Kit contains | IR (β-subunit) Standard, Diluents, Detector Antibody, Secondary Antibody, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. |
| Positive control | IR (β-subunit) |
Biological Information
| Assay range | 0.94-60 ng/ml |
| Assay time | 4 h |
| Sample Type | Cells |
Physicochemical Information
| Sensitivity | < 0.5 ng/ml |
Product Usage Statements
| Intended use | The Calbiochem® Insulin Receptor (β-subunit) ELISA Kit is designed to detect and quantify levels of the insulin receptor β-subunit independent of its phosphorylation status. Both natural (heterotetrameric) and recombinant IR react in this assay. This ELISA is not cross-reactive with IGF-1R. Although this ELISA kit is developed using cells containing human insulin receptor, mouse and rat insulin receptor will cross react. This assay is intended for detection of IR from lysates of cells. |
Storage and Shipping Information
| Ship Code | Blue Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | +2°C to +8°C |
| Storage Conditions | Upon arrival store the entire contentsof the kit at 4°C. |
| Do not freeze | Ok to freeze |
