Lugol's Solution 1Ltr
for the Gram staining method
Manufacturer: Merck
Catalog ID: 109261.1000
Stock: In Stock
Description
| Catalogue Number | 109261 |
| Synonyms | Iodine-potassium iodide solution |
| Description | LUGOL'S SOLUTION (DILUTED IODINE-POTASS |
| Overview | In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with Gram's iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear orange. A 500 mL PE-bottle of Gram's Lugol's solution is sufficient for staining up to 250 slides. Lugol’s solution is also a part of the Gram-color stain set. It is an IVD & CE product. |
Product Information
| HS Code | 3822 00 00 |
Physicochemical Information
| Boiling point | 100 °C (1013 hPa) |
| Density | 1.01 g/cm3 (20 °C) |
| pH value | 3.5 (H₂O, 20 °C) |
Storage and Shipping Information
| Storage | Store at +15°C to +25°C. |
Specifications
| Suitability for microscopy | passes test |
| gram-positve microorganisms | blue-violet |
| gram-negative microorganisms | pink to red |
