Methylene Blue 25gm
for microscopy Certistain®
Manufacturer: Merck
Catalog ID: 115943.0025
Stock: In Stock
Description
| Catalogue Number | 115943 |
| Synonyms | Methylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride |
| Description | METHYLENE BLUE (C.I. 52015) FOR MICROSCO |
| Overview | Methylene blue (C.I. 52015) is a Certistain® dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration. |
Product Information
| EC number | 200-515-2 |
| Hill Formula | C₁₆H₁₈ClN₃S * x H₂O |
| Chemical formula | C₁₆H₁₈ClN₃S * x H₂O (x=2-3) |
| Molar Mass | 319.86 g/mol (anhydrous) |
| HS Code | 3204 13 00 |
Physicochemical Information
| Melting Point | 180 °C (decomposition) |
| pH value | 3 (10 g/l, H₂O, 20 °C) |
| Bulk density | 400 - 600 kg/m3 |
| Solubility | 50 g/l |
Toxicological Information
| LD 50 oral | LD50 Rat 1180 mg/kg |
Storage and Shipping Information
| Storage | Store at +5°C to +30°C. |
Specifications
| Dye content (spectrophotometrically) | ≥ 82 % |
| Identity - Chloride | passes test |
| - UV/VIS-Spectrum | passes test |
| Absorption maximum λ max (ethanol 50 %) | 660 - 665 nm |
| Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %) | 2250 - 2750 |
| TLC-Test | passes test |
| Loss on drying (110 °C) | 10 - 15 % |
| Suitability for microscopy | passes test |
