Plate Count Agar 500grm
acc. ISO 4833, ISO 17410 and FDA-BAM GranuCult™
Manufacturer: Merck
Catalog ID: 105463.0500
Stock: In Stock
Description
| Catalogue Number | 105463 |
| Replaces | 1.05463.5007 |
| Synonyms | Casein-peptone dextrose yeast agar |
| Overview | GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly. |
Product Information
| HS Code | 3821 00 00 |
Physicochemical Information
| pH value | 7.0 (22 g/l, H₂O, 30 °C) (after autoclaving) |
| Bulk density | 560 kg/m3 |
| Solubility | 22.5 g/l |
Storage and Shipping Information
| Storage | Store at +15°C to +25°C. |
Specifications
| Appearance (clearness) | clear |
| Appearance (colour) | yellowish to yellowish-brown |
| Solidification behaviour (2 hrs., 40 °C) | liquid |
| Solidification behaviour (4 hrs., 45 °C) | liquid |
| pH-value (25 °C) | 6.8 - 7.2 |
| Growth promotion test in accordance with the current version of DIN EN ISO 11133. | |
| Incubation: 72 ± 3 hours at 30 ± 1 °C; aerobic | |
| Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)) | |
| Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)) | |
| Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) | |
| Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) | |
| Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003)) | |
| Inoculum on reference medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)) | 10 - 100 |
| Inoculum on reference medium (Listeria monocytogenes ATCC 19118) | 10 - 100 |
| Inoculum on reference medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)) | 10 - 100 |
| Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)) | |
| Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)) | |
| Colony count (Escherichia coli ATCC 8739 (WDCM 00012)) | |
| Colony count (Escherichia coli ATCC 25922 (WDCM 00013)) | |
| Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003)) | |
| Colony count (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)) | |
| Colony count (Listeria monocytogenes ATCC 19118) | |
| Colony count (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)) | |
| Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)) | ≥ 70 % |
| Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)) | ≥ 70 % |
| Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)) | ≥ 70 % |
| Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)) | ≥ 70 % |
| Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003)) | ≥ 70 % |
| Recovery on test medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)) | ≥ 70 % |
| Recovery on test medium (Listeria monocytogenes ATCC 19118) | ≥ 70 % |
| Recovery on test medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)) | ≥ 70 % |
| Incubation: 24 hours at 35 - 37 °C; aerobic | |
| Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)) | |
| Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)) | |
| Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) | |
| Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) | |
| Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003)) | |
| Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)) | |
| Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)) | |
| Colony count (Escherichia coli ATCC 8739 (WDCM 00012)) | |
| Colony count (Escherichia coli ATCC 25922 (WDCM 00013)) | |
| Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003)) | |
| Recovery (Staphylococcus aureus ATCC 6538 (WDCM 00032)) | ≥ 70 % |
| Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)) | ≥ 70 % |
| Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)) | ≥ 70 % |
| Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)) | ≥ 70 % |
| Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003)) | ≥ 70 % |
| Reference: Tryptic soy agar.S.aureus, E.coli and B.subtilis tested with pour plate technic.A recovery rate of 70 % is equivalent to a productivity value of 0.7.The indicated colony counts result from the sum of a triple determination.Other strains in single determination with spiral platemethode. |
